dc.contributor.author | Purdue, Laura | |
dc.date.accessioned | 2020-01-21T19:15:31Z | |
dc.date.available | 2020-01-21T19:15:31Z | |
dc.date.issued | 2020-01-21T19:15:31Z | |
dc.identifier.uri | http://hdl.handle.net/10222/77298 | |
dc.description.abstract | The aim of this research was to isolate and characterize marine thraustochytrids to find a strain that could potentially be used to produce squalene in industrial fermentations. To support this goal, a method for laboratory-scale high throughput squalene extraction was developed to aid in screening the large number of isolates discovered in this work. A total of 406 marine isolates were found from four sampling trips, 270 of which were confirmed as thraustochytrids, based on their morphology. Through a series of flask level screening steps, the best strain for squalene production was selected and used for media development. Media factor screening using a Plackett-Burman design produced a maximum squalene content and concentration of 63.94 mg g-1 and 665.96 mg L-1, respectively. The three media factors that had the largest positive effect on squalene production were glycerol, sodium chloride and temperature. | en_US |
dc.language.iso | en | en_US |
dc.subject | Thraustochytrid | en_US |
dc.subject | Squalene | en_US |
dc.subject | Bioprospecting | en_US |
dc.subject | Extraction | en_US |
dc.title | The Isolation of Marine Organisms for the Sustainable Production of Squalene | en_US |
dc.date.defence | 2018-12-07 | |
dc.contributor.department | Department of Process Engineering and Applied Science | en_US |
dc.contributor.degree | Master of Applied Science | en_US |
dc.contributor.external-examiner | n/a | en_US |
dc.contributor.graduate-coordinator | Dr. Azadeh Kermanshahi pour | en_US |
dc.contributor.thesis-reader | Dr. Sue Budge | en_US |
dc.contributor.thesis-reader | Dr. Azadeh Kermanshahi pour | en_US |
dc.contributor.thesis-supervisor | Dr. Su-Ling Brooks | en_US |
dc.contributor.thesis-supervisor | Dr. Roberto Armenta | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |