dc.contributor.author | Daurie, Angela | |
dc.date.accessioned | 2016-08-02T15:32:16Z | |
dc.date.available | 2016-08-02T15:32:16Z | |
dc.date.issued | 2016-08-02T15:32:16Z | |
dc.identifier.uri | http://hdl.handle.net/10222/72025 | |
dc.description.abstract | Shigella flexneri is the causative agent of dysentery. An oral murine infection model, that uses the broad-spectrum antibiotic streptomycin prior to Shigella infection, has been established and displays hallmarks of Shigella infection in humans.
I used streptomycin pretreated BALB/c mice to characterize the role of OspG during infection. Shigella mutant ΔospG causes 30% mortality accompanied by a change in the gross pathology of the cecum and colon in these mice. Mortality returned to wild type infection levels when I complemented with WT OspG but not in mice infected with the ΔospGmut that does not interact with the host E2-ubiqutin conjugate.
I infected a variety of mouse strains and found differences in susceptibility. I was able to show that host factors, bacteria effectors and facility differences play a role in disease severity to Shigella infection. I was successful in expanding our knowledge about the streptomycin mouse model. | en_US |
dc.language.iso | en | en_US |
dc.subject | Shigella | en_US |
dc.subject | OspG | en_US |
dc.subject | Animal models for Shigellosis | en_US |
dc.subject | disease severity | en_US |
dc.subject | streptomycin mouse model | en_US |
dc.subject | Models, Animal. | |
dc.title | APPLICATIONS AND CHARACTERIZATION OF A STREPTOMYCIN MOUSE MODEL FOR SHIGELLOSIS | en_US |
dc.date.defence | 2016-05-13 | |
dc.contributor.department | Department of Microbiology & Immunology | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.external-examiner | Nikhil Thomas | en_US |
dc.contributor.graduate-coordinator | Craig McCormick | en_US |
dc.contributor.thesis-reader | Brent Johnston | en_US |
dc.contributor.thesis-reader | Andrew Stadnyk | en_US |
dc.contributor.thesis-supervisor | John Rohde | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.manuscripts | Yes | en_US |
dc.contributor.copyright-release | Yes | en_US |