dc.contributor.author | Blundon, Heather L. | |
dc.date.accessioned | 2015-08-21T14:32:25Z | |
dc.date.available | 2015-08-21T14:32:25Z | |
dc.date.issued | 2015 | |
dc.identifier.uri | http://hdl.handle.net/10222/60764 | |
dc.description.abstract | Chemerin is a fat tissue-secreted protein that activates cellular processes though chemokine-like receptor 1 (CMKLR1) signalling. Serum chemerin is positively correlated to atherosclerosis severity, however the functional role of chemerin in atherosclerosis remains unknown. The study goal was to assess the role of chemerin/CMKLR1 signalling in human and mouse vascular smooth muscle cell (VSMC) migration and proliferation (important stages of atherosclerotic plaque formation). A protocol for differentiating human adipose-derived stem cells into SMCs was investigated. Morphological VSMC-characteristics were observed, as was chemerin and CMKLR1 expression, however further VSMC phenotype confirmation is required. Therefore, a second approach used primary aortic mouse VSMCs from CMKLR1-expressing and CMKLR1-deficient mice. Mouse VSMCs expressed chemerin and CMKLR1, and secreted low levels of chemerin. Low chemerin concentrations enhanced VSMC migration via CMKLR1 signalling and high concentrations inhibited migration via a CMKLR1-independent mechanism. This data suggests the involvement of chemerin/CMKLR1 signalling in atherogenic VSMC processes. | en_US |
dc.language.iso | en | en_US |
dc.subject | Chemerin | en_US |
dc.subject | Atherosclerosis | en_US |
dc.subject | Vascular Smooth Muscle Cell (VSMC) | en_US |
dc.title | Development of vascular smooth muscle cell models for investigating the role of CMKLR1/chemerin signalling in atherosclerosis | en_US |
dc.type | Thesis | en_US |
dc.date.defence | 2015-08-12 | |
dc.contributor.department | College of Pharmacy | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.external-examiner | N/A | en_US |
dc.contributor.graduate-coordinator | Dr. David Jakeman | en_US |
dc.contributor.thesis-reader | Dr. Roger McLeod | en_US |
dc.contributor.thesis-reader | Dr. Tannis Jurgens | en_US |
dc.contributor.thesis-supervisor | Dr. Chris Sinal | en_US |
dc.contributor.thesis-supervisor | Dr. Kerry Goralski | en_US |
dc.contributor.ethics-approval | Received | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |