dc.contributor.author | Sukumar, Vaishnavi | |
dc.date.accessioned | 2015-04-29T17:45:38Z | |
dc.date.available | 2015-04-29T17:45:38Z | |
dc.date.issued | 2015-04-29 | |
dc.identifier.uri | http://hdl.handle.net/10222/56744 | |
dc.description.abstract | Efferent neurons containing GABA, glutamate, acetylcholine and octopamine (OA)
innervate spider, Cupiennius salei, mechanosensory neurons. The spider leg transcriptome
library contains several putative transmitter receptors including G-protein coupled α- and
β-adrenergic OA and tyramine (TA) receptors.
I performed intracellular recordings from the strain detecting VS-3 neurons. TA
caused a dose-dependent rise in action potential firing with an EC50 of 15.1 μM. VS-3
neuron sensitivity increased at all frequencies, but particularly in the high frequency range.
Mianserin, an antagonist of β-adrenergic-like receptors, blocked the TA effect. Yohimbine,
an antagonist of α-adrenergic-like receptors did not inhibit the TA effect, but reduced the
baseline firing. These results indicate that the VS-3 neurons have at least two different
adrenergic receptor types. The effects of TA and OA are similar, although OA is more
potent. Thus, TA probably activates β-adrenergic OA receptors. The yohimbine effect
indicates that α-adrenergic receptors maintain baseline sensitivity in VS-3 neurons. | en_US |
dc.language.iso | en | en_US |
dc.subject | Spider | en_US |
dc.subject | Cupiennius | |
dc.subject | salei | |
dc.subject | arthropod | |
dc.subject | arachnid | |
dc.subject | tyramine | |
dc.subject | mechanoreceptors | |
dc.subject | octopamine | |
dc.subject | VS-3 | |
dc.subject | Lyriform Slit Sensilla | |
dc.subject | Electrophysiology | |
dc.title | THE ROLE OF TYRAMINE IN MODULATION OF SPIDER, CUPIENNIUS SALEI, MECHANORECEPTORS | en_US |
dc.date.defence | 2015-04-28 | |
dc.contributor.department | Department of Physiology & Biophysics with Neuroscience | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.external-examiner | Dr. Shelly Adamo | en_US |
dc.contributor.graduate-coordinator | Dr. Robert Rose | en_US |
dc.contributor.thesis-reader | Dr. Kazue Semba | en_US |
dc.contributor.thesis-reader | Dr. Stefan Krueger | en_US |
dc.contributor.thesis-supervisor | Dr. Paivi H Torkkeli | en_US |
dc.contributor.thesis-supervisor | Dr. Andrew S French | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | No | en_US |