dc.contributor.author | Wood, Leah M. | |
dc.date.accessioned | 2011-01-18T15:14:56Z | |
dc.date.available | 2011-01-18T15:14:56Z | |
dc.date.issued | 2011-01-18 | |
dc.identifier.uri | http://hdl.handle.net/10222/13187 | |
dc.description.abstract | Electroretinoraphic signals (ERGs) are affected when recorded under isoflurane anesthesia in the operating room. We explored the effect of isoflurane and propofol in ex vivo guinea pig retinal preparations using a multielectrode array to record simultaneously ERGs and retinal ganglion cell (RGC) activity. The viability and light-response characteristics of the model were documented. In the presence of isoflurane, the ERG and RGC activity was reduced in a dose-dependent manner, even at sub-clinical doses; the OFF responses were consistently more affected. Propofol had minimal effects: at subclinical doses, a small excitation was measured while a concentration a hundred times stronger than the clinical concentration was required to measure a significant decline in EGR and RGC signals. This study confirms the usefulness of the guinea pig model to study clinically relevant retinal issues and shows that propofol is a better anesthetic to use in the operating room when retinal investigations are required. | en_US |
dc.language.iso | en | en_US |
dc.subject | electroretinogram | en_US |
dc.subject | isoflurane | en_US |
dc.subject | propofol | en_US |
dc.subject | multielectrode array | en_US |
dc.subject | retinal activity | en_US |
dc.title | REGULATION OF RETINAL ACTIVITY IN AN EX-VIVO GUINEA PIG MODEL BY EXPERIMENTAL CONDITIONS AND EFFECTS OF ISOFLURANE AND PROPOFOL ANESTHETICS | en_US |
dc.date.defence | 2010-10-21 | |
dc.contributor.department | Department of Clinical Vision Science | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.external-examiner | Dr. Sarah Stevens | en_US |
dc.contributor.graduate-coordinator | Dr. Balwantray Chauhan | en_US |
dc.contributor.thesis-reader | Heather Fennell | en_US |
dc.contributor.thesis-reader | Dr. Gautam Awatramani | en_US |
dc.contributor.thesis-supervisor | Dr. Francois Tremblay | en_US |
dc.contributor.ethics-approval | Received | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |