Browsing by Subject "Mass Spectrometry"
Now showing items 1-7 of 7
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ACYL CARRIER PROTEIN DYNAMICS AND PARTNER ENZYME INTERACTIONS
(2015)Acyl carrier protein (ACP) is essential for the synthesis of fatty acids, phospholipids, lipid A and other primary and secondary metabolites. ACP and ACP-like proteins are found throughout nature; however, differences ... -
Assessment, Development, and Improvement of SDS Depletion Strategies in Mass Spectrometry Proteomic Workflows
(2016-08-22)In proteomics, sodium dodecyl sulfate negatively affects trypsin digestion, chromatography, and mass spectrometry, thus, its effective depletion is critical to any protein sample workflow. A quantitative assessment of SDS ... -
Development of Techniques for Removal of Sodium Dodecyl Sulfate for Enhanced Protein Identification and Characterization
(2011-12-13)Mass spectrometry is a powerful tool employed in proteomics; however, sodium dodecyl sulfate (SDS), a surfactant used for protein solubilization, is known to cause severe interference at concentrations greater than 0.01%. ... -
Electrospray Ionization Tandem-MS of Ammonium and Alkali Metals of TAGs and their Oxidation Products with Fractionation by LC-MS and FAIMS
(2022-08-22)Triacylglycerols (TAGs) are the main component of edible oils, where unsaturated TAGs are easily oxidized, leading to toxicity and negative health effects. The analysis of TAG and TAG oxidation products is an ongoing ... -
Methods to Isolate Proteins from Detergent-containing Solutions for Proteome Analysis
(2014-12-10)The use of the detergent sodium dodecyl sulfate (SDS) to assist in the solubilization of protein samples can be highly beneficial in the proteomics workflow. However, SDS is incompatible with LC-MS. Acetone precipitation ... -
Site-Specific Tyrosine Phosphorylation of the Type III Secretion Chaperone CesT Regulates Effector Hierarchy and Promotes Enteropathogenic Escherichia coli Intestinal Disease
(2017-08-22)Enteropathogenic E. coli (EPEC) is a global enteric pathogen that causes serious gastrointestinal disease and inflicts a significant burden on healthcare systems worldwide. Recent phosphoproteomic studies suggest that ... -
TOWARDS BIOMARKER DISCOVERY IN CONGENITAL URINARY TRACT OBSTRUCTION
(2014-05-30)Proteome analysis techonologies are commonly employed for discovery-based biomarker identification studies. This thesis aims to help bridge the gap between analytical technology development and clinical application by ...