dc.contributor.author | Ridley, Christina M | |
dc.date.accessioned | 2012-07-23T12:51:31Z | |
dc.date.available | 2012-07-23T12:51:31Z | |
dc.date.issued | 2012-07-23 | |
dc.identifier.uri | http://hdl.handle.net/10222/15091 | |
dc.description.abstract | Waterborne pathogen presence caused by fecal pollution is a leading cause of morbidity and mortality worldwide. In developed countries, this problem can result in waterborne outbreaks. Research suggests that there is a need for better fecal indicators because current methods (total coliforms and E. coli) are insufficient. This study investigated Bacteroidales 16S rRNA markers as a microbial source tracking tool in an agricultural watershed. Correlations between pathogens and markers were also investigated. Water quality monitoring was conducted following assay validation of ruminant-, bovine-, human-specific, and universal Bacteroidales markers. Results revealed a positive relationship between E. coli and the universal marker. Ruminant- and bovine-specific marker detection was associated with increased runoff due to precipitation; however, the human associated marker was not detected. Furthermore, no correlations between Campylobacter, Salmonella, or E. coli O157:H7 could be made. Consequently, these techniques have potential to become a powerful tool; however, further research is needed | en_US |
dc.language.iso | en | en_US |
dc.subject | Bacteroidales | en_US |
dc.subject | Microbial Source Tracking | en_US |
dc.subject | Water Quality | en_US |
dc.subject | Waterborne pathogens | en_US |
dc.subject | E. coli O157:H7 | en_US |
dc.subject | Salmonella | en_US |
dc.subject | Campylobacter | en_US |
dc.subject | Fecal indicator organisms | en_US |
dc.title | Evaluation of Bacteroidales 16S rRNA Genetic Markers as a Microbial Source Tracking Tool in a Canadian Agricultural Watershed | en_US |
dc.date.defence | 2012-06-15 | |
dc.contributor.department | Department of Process Engineering and Applied Science | en_US |
dc.contributor.degree | Master of Applied Science | en_US |
dc.contributor.external-examiner | N/A | en_US |
dc.contributor.graduate-coordinator | Georges Kipouros | en_US |
dc.contributor.thesis-reader | Greg Bezanson | en_US |
dc.contributor.thesis-reader | Chris Yost | en_US |
dc.contributor.thesis-supervisor | Rob Jamieson; Lisbeth Truelstrup Hansen | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |